BRCA1 antibody (pSer1189)

Catalog No. ABIN127308

The Rabbit Polyclonal anti-BRCA1 antibody (ABIN127308) specifically detects BRCA1 in WB. The antibody is reactive with Human samples.

Quick Overview for BRCA1 antibody (pSer1189) (ABIN127308)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRCA1 Antibody

Binding Specificity: pSer1189

Specificity: Detect BRCA1 when phosphorylated at Ser1189.

Purification: Peptide affinity purified

Immunogen: Phosphorylated synthetic peptide surrounding Serine 1189 of human BRCA1.

Application Details

Application Notes: Western Blot: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 / RNF53

Background: BRCA1 is a breast cancer susceptibility gene that was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45 % of families with high incidence of breast cancer and at least 80 % of families with increased incidence of both earlyonset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. Like many other genes involved in familial cancer, BRCA1 appears to encode a tumor suppressor, a protein that acts as a negative regulator of tumor growth.Synonyms: Breast cancer type 1 susceptibility protein, RING finger protein 53

Gene ID: 672

NCBI Accession: NP_009225

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus