GFP antibody

Catalog No. ABIN129564

Supplier Product No.: 600-301-215

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB, ELISA, IHC, IP, FM, FACS and DB. Suitable for Aequorea victoria. This Primary Antibody has been cited in 17+ publications.

Quick Overview for GFP antibody (ABIN129564)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Flow Cytometry (FACS), Dot Blot (DB)

Clone: 9F9-F9

Product Details anti-GFP Antibody

Supplier Product No.: 600-301-215

Supplier: Rockland

Purpose: GFP Monoclonal Antibody

Cross-Reactivity (Details): Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.

Characteristics: Synonyms: mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:3,000 - 1:30,000

ELISA Dilution: 1:10,000 - 1:30,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN129564)

Žaja, Aydin, Lippok, Feederle, Lüscher, Feijs: "Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome." in: Scientific reports, Vol. 10, Issue 1, pp. 8286, (2020) (PubMed).

Santos, Damásio, Kun-Rodrigues, Barbot, Sequeiros, Brás, Alonso, Guerreiro: "Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype." in: Journal of clinical medicine, Vol. 9, Issue 4, (2020) (PubMed).

Dohmen, Krieg, Agalaridis, Zhu, Shehata, Pfeiffenberger, Amelang, Bütepage, Buerova, Pfaff, Chanda, Geley, Preisinger, Sakamoto, Lüscher, Neumann, Vervoorts: "AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy." in: Nature communications, Vol. 11, Issue 1, pp. 1032, (2020) (PubMed).

Santos, Morais, Pereira, Sequeiros, Alonso: "Parkin truncating variants result in a loss-of-function phenotype." in: Scientific reports, Vol. 9, Issue 1, pp. 16150, (2019) (PubMed).

Khlghatyan, Evstratova, Chamberland, Marakhovskaia, Bahremand, Toth, Beaulieu: "Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3β Are Modulators of Anxiety and Glutamatergic Neurotransmission." in: Frontiers in molecular neuroscience, Vol. 11, pp. 119, (2018) (PubMed).

Terrell, Wu, Tsao, Barber, Servinsky, Payne, Bentley: "Nano-guided cell networks as conveyors of molecular communication." in: Nature communications, Vol. 6, pp. 8500, (2016) (PubMed).

Moerdyk-Schauwecker, Shah, Murphy, Hastie, Mukherjee, Grdzelishvili: "Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling." in: Virology, Vol. 436, Issue 1, pp. 221-34, (2013) (PubMed).

Feijs, Kleine, Braczynski, Forst, Herzog, Verheugd, Linzen, Kremmer, Lüscher: "ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation." in: Cell communication and signaling : CCS, Vol. 11, Issue 1, pp. 5, (2013) (PubMed).

Hastie, Besmer, Shah, Murphy, Moerdyk-Schauwecker, Molestina, Roy, Curry, Mukherjee, Grdzelishvili: "Oncolytic vesicular stomatitis virus in an immunocompetent model of MUC1-positive or MUC1-null pancreatic ductal adenocarcinoma." in: Journal of virology, Vol. 87, Issue 18, pp. 10283-94, (2013) (PubMed).

Forst, Karlberg, Herzog, Thorsell, Gross, Feijs, Verheugd, Kursula, Nijmeijer, Kremmer, Kleine, Ladurner, Schüler, Lüscher: "Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains." in: Structure (London, England : 1993), Vol. 21, Issue 3, pp. 462-75, (2013) (PubMed).

Goel, Sienkiewicz, Picciani, Wang, Lee, Bhattacharya: "Cochlin, intraocular pressure regulation and mechanosensing." in: PLoS ONE, Vol. 7, Issue 4, pp. e34309, (2012) (PubMed).

Goel, Sienkiewicz, Picciani, Lee, Bhattacharya: "Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility." in: PLoS ONE, Vol. 6, Issue 8, pp. e23070, (2011) (PubMed).

Jesse, Koenig, Ellenrieder, Menke: "Lef-1 isoforms regulate different target genes and reduce cellular adhesion." in: International journal of cancer, Vol. 126, Issue 5, pp. 1109-20, (2010) (PubMed).

Shen, Da Silva, He, Cline: "Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 15, pp. 5032-43, (2009) (PubMed).

Forrest, Paden, Allen, Collins, Speck: "ORF73-null murine gammaherpesvirus 68 reveals roles for mLANA and p53 in virus replication." in: Journal of virology, Vol. 81, Issue 21, pp. 11957-71, (2007) (PubMed).

Yang, Camp, Gritsenko, Luo, Kelly, Clauss, Brinkley, Smith, Stenoien: "Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 4060-70, (2007) (PubMed).

Koenig, Mueller, Hasel, Adler, Menke: "Collagen type I induces disruption of E-cadherin-mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells." in: Cancer research, Vol. 66, Issue 9, pp. 4662-71, (2006) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212