CD8 antibody (PE,Cy5)

Catalog No. ABIN133193

Product Details anti-CD8 Antibody (PE,Cy5)

Target: CD8

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to PE,Cy5

Application: Flow Cytometry (FACS)

Specificity: Rat CD8a

Purification: Ion exchange

Clone: OX-8

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/ml

Storage: 4 °C

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: Anti-rat CD8a monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine. (1,2,3,4.) The antigen recognized is a complex of surface glycoproteins of Mr 34,000, 39,000 and 76,000 Da and is the rat homologue of the human CD8 and the mouse Ly-2,3 antigen. (5,8) ABIN133193labels all peripheral T cells that are unlabelled by the ABIN000000 (W3/25) monoclonal antibody. It labels a T-cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor. (2) ABIN133193and ABIN000000 can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft vs. host disease. (7) This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being ABIN000000 (W3/13) which labels all T cells, as well as ABIN000000 (W3/25) and CL004TC(MRC 0X-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes.