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- Target
- CD45RA Or A/B
- Reactivity
- Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS)
- Specificity
- Rat CD45RA/B
- Purification
- Purified from ascitic fluid via Protein G Chromatography
- Immunogen
- Rat purified spleen L-CA . Fusion Partner: NSO/U . Donor: BALB/c spleen
- Clone
- OX-33
- Isotype
- IgG1
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- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/ml
- Buffer
- Purified Ig buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography)
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- CD45RA Or A/B
- Background
- The rat CD45 antigen (Leukocyte Common Antigen (L-CA)) has been shown to exist in different forms on different lymphoid cell types. CD45 on thymocytes shows one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa. ABIN133310detects a subfraction of the 240 kDa rat CD45 band found only on B lymphocytes. This monoclonal antibody is of particular use in the investigation of the molecular and antigenic heterogeneity of rat L-CA, especially when used in conjunction with other OX antibodies. Four mouse monoclonal antibodies (MRC OX 1, 28, 29 and 30) react with all molecular forms of L-CA and fall into two sets that are non-competitive in binding to L-CA (MRC OX 1, 28, 29 and 30). The antigenic determinants seen by all these antibodies are lost when L-CA is reduced and alkylated. Three antibodies (MRC OX 22, 31, and 32) react selectively with B cells, T cytotoxic cells and about two thirds of T helper cells. OX 22 and OX 31 compete for binding but are non- competitive with OX 32. All of these antibodies bind to a sub-fraction of the 190, 200 and 220 kDa forms of T cell L-CA but not at all to the 180 kDa form of T cells or thymocytes. This clone works in flow cytometry and with frozen and paraffin sections (2).
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