CD45RA/B antibody (Biotin)
CD45RA/B
Reactivity: Rat
FACS
Host: Mouse
Monoclonal
OX-33
Biotin
-
- Target See all CD45RA/B products
- CD45RA/B
- Reactivity
- Rat
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CD45RA/B antibody is conjugated to Biotin
- Application
- Flow Cytometry (FACS)
- Specificity
- Rat CD45RA/B
- Purification
- Ion exchange chromatography
- Immunogen
- Rat purified spleen L-CA . Fusion Partner: NSO/U . Donor: BALB/c Spleen
- Clone
- OX-33
- Isotype
- IgG1
-
-
- Application Notes
- This clone is reported to work with frozrn and paraffin sections (2).
- Restrictions
- For Research Use only
-
- Concentration
- 0.5 µg/10 cells
- Storage
- 4 °C
-
- Target
- CD45RA/B
- Alternative Name
- CD45RA/B (CD45RA/B Products)
- Background
- Rat Leukocyte Common Antigen has been shown to exist in different forms on different lymphoid cell types. Rat L-CA on thymocytes shows one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa. ABIN133312detects a subfraction of the 240 kDa rat L-CA band found only on B lymphocytes. This monoclonal antibody is of particular use in the investigation of the molecular and antigenic heterogeneity of rat L-CA, especially when used in conjunction with other OX antibodies. Four mouse monoclonal antibodies (MRC OX 1, 28, 29 and 30) react with all molecular forms of L-CA and fall into two sets that are non- competitive in binding to L-CA (MRC OX 1, 28, 29 and 30). The antigenic determinants seen by all these antibodies are lost when L-CA is reduced and alkylated. Three antibodies (MRC OX 22, 31, and 32) react selectively with B cells, T cytotoxic cells and about two thirds of T helper cells. OX 22 and OX 31 compete for binding but are non- competitive with OX 32. All of these antibodies bind to a sub-fraction of the 190, 200 and 220 kDa forms of T cell L-CA but not at all to the 180 kDa form of T cells or thymocytes. This clone is reported to work with frozrn and paraffin sections (2).
-
You are here:
