COL2 antibody

Catalog No. ABIN135048

Cited in 7+ publications. The Goat Polyclonal anti-COL2 antibody (ABIN135048) specifically detects COL2 in WB, ELISA, IP, ICC, IHC (p), IHC (fro) and IEM. The antibody is reactive with Human, Mouse, Rat, Rabbit, Chicken, Cow, Sheep and Pig samples.

Quick Overview for COL2 antibody (ABIN135048)

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Cow, Sheep, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This COL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Product Details anti-COL2 Antibody

Purpose: Goat Anti-Type II Collagen-UNLB

Specificity: Reacts with conformational determinants on type II collagen Referenced species reactivities include - Human 4,5,10,12,14,18-20,22,24,27,29,31,33,34 Chicken 16,26 Mouse 6,7,15 Porcine 21 Rat 13,17,25 Rabbit 2,9,11,30,32 Bovine 2,3,19,23 Sheep 8,28

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Purified Anti-Type II Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, and immunoprecipitation assays.

Purification: Purified by affinity chromatography on type II collagen covalently linked to agarose, cross-adsorbed against Collagen types I, III, IV, V, and VI for minimal reactivity

Immunogen: Type II collagen

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with type II collagen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Borate buffered saline, pH 8.2

Storage: 4 °C

Storage Comment: 2-8°C

References for anti-COL2 antibody (ABIN135048)

Tan Timur, Caron, van den Akker, van der Windt, Visser, van Rhijn, Weinans, Welting, Emans, Jahr: "Increased TGF-β and BMP Levels and Improved Chondrocyte-Specific Marker Expression In Vitro under Cartilage-Specific Physiological Osmolarity." in: International journal of molecular sciences, Vol. 20, Issue 4, (2019) (PubMed).

Kuwahara, Serowoky, Vakhshori, Tripuraneni, Hegde, Lieberman, Crump, Mariani: "Sox9+ messenger cells orchestrate large-scale skeletal regeneration in the mammalian rib." in: eLife, Vol. 8, (2019) (PubMed).

Fujii, Hirota, Yasoda, Takizawa, Morozumi, Nakamura, Yotsumoto, Kondo, Yamashita, Sakane, Kanai, Ueda, Yamauchi, Yamanaka, Nakao, Kuwahara, Jindo, Furuya, Mashimo, Inagaki, Serikawa, Nakao: "Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death." in: PLoS ONE, Vol. 13, Issue 3, pp. e0194812, (2018) (PubMed).

Caron, Emans, Sanen, Surtel, Cremers, Ophelders, van Rhijn, Welting: "The Role of Prostaglandins and COX-Enzymes in Chondrogenic Differentiation of ATDC5 Progenitor Cells." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153162, (2016) (PubMed).

Nakao, Osawa, Yasoda, Yamanaka, Fujii, Kondo, Koyama, Kanamoto, Miura, Kuwahara, Akiyama, Bessho, Nakao: "The Local CNP/GC-B system in growth plate is responsible for physiological endochondral bone growth." in: Scientific reports, Vol. 5, pp. 10554, (2016) (PubMed).

van den Akker, Surtel, Cremers, Rodrigues-Pinto, Richardson, Hoyland, van Rhijn, Welting, Voncken: "Novel immortal human cell lines reveal subpopulations in the nucleus pulposus." in: Arthritis research & therapy, Vol. 16, Issue 3, pp. R135, (2014) (PubMed).

Mirmalek-Sani, Orlando, McQuilling, Pareta, Mack, Salvatori, Farney, Stratta, Atala, Opara, Soker: "Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering." in: Biomaterials, Vol. 34, Issue 22, pp. 5488-95, (2013) (PubMed).

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: TypeII Collagen

Background:

Description: Goat Anti-Type II Collagen-UNLB

NCBI_Official_Symbol: COL2A1

Synoynms: COL2

Gene ID: 1280

UniProt: P02458