COL2 antibody (CII-J1 epitope)

Catalog No. ABIN2191818

Product Details anti-COL2 Antibody

Target: COL2 (Collagen, Type II (COL2))

Binding Specificity: CII-J1 epitope

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Detection (D), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: M2139

Application Details

Application Notes: For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS and 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Collagen Type II (COL2 Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, CAP18 antibody, Cnlp antibody, Cramp antibody, FALL39 antibody, MCLP antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, cathelicidin antimicrobial peptide antibody, Cuticle collagen 2 antibody, collagen type II alpha 1 chain antibody, F2 antibody, Camp antibody, col-2 antibody, COL2A1 antibody

Background: The monoclonal antibody M2139 reacts with the J1 epitope (triple helical position 551-564) of collagen type II. Collagen is a structural protein in bone, cartillage and connective tissue. Collagen type II (CII) is the major collagen of the nucleus pulposa (a component of spine), cartilage and vitreous (a component of the eye). The most commonly used animal model for rheumatoid arthritis (RA) is the collagen-induced arthritis (CIA). Transfer of collagen type II specific monoclonal antibodies induces an acute form of arthritis (collagen type II antibody induced arthritis, CAIA). The monocolonal antibody M2139 has been shown to induce CAIA in naive mice after injection of lipopolysacharide (LPS). However, in combination with the monoclonal antibody CIIC1, binding to the C1 epitope of CII, the pair of monoclonal antibodies induce arthritis in different strains of mice without any other stimulants. The presence of secondary stimulus, LPS, increases the disease incidence and severity. The monoclonal antibody M2139 is cross reactive with rat, bovine, chicken and human CII.