COL2 antibody (Biotin)

Catalog No. ABIN135049

This Goat Polyclonal antibody specifically detects COL2 in WB, ELISA, IP, ICC, IHC (p), IHC (fro) and IEM. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Chicken, Cow, Sheep and Pig.

Quick Overview for COL2 antibody (Biotin) (ABIN135049)

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Cow, Sheep, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This COL2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Product Details anti-COL2 Antibody (Biotin)

Purpose: Goat Anti-Type II Collagen-BIOT

Specificity: Reacts with conformational determinants on type II collagen Referenced species reactivities include - Human 4,5,10,12,14,18-20,22,24,27,29,31,33,34 Chicken 16,26 Mouse 6,7,15 Porcine 21 Rat 13,17,25 Rabbit 2,9,11,30,32 Bovine 2,3,19,23 Sheep 8,28

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Biotin Anti-Type II Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, and immunoprecipitation assays.

Purification: Purified by affinity chromatography on type II collagen covalently linked to agarose, cross-adsorbed against Collagen types I, III, IV, V, and VI for minimal reactivity

Immunogen: Type II collagen

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with type II collagen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Type II Collagen

Background:

Description: Goat Anti-Type II Collagen-BIOT

NCBI_Official_Symbol: COL2A1

Synoynms: COL2

Gene ID: 1280

UniProt: P02458