Collagen IV antibody (Biotin)

Catalog No. ABIN135053

There are 2+ publications for this product available. The Goat Polyclonal anti-Collagen IV antibody is suitable to detect Collagen IV in samples from Human, Mouse, Rat, Pig, Rabbit, Chicken, Chinchilla chinchilla, Dog, Guinea Pig, Horse, Japanese macaque (Macaca fuscata), Sheep and Tree shrew. It has been validated for WB, ELISA, IHC (fro), IHC (p), FACS, IP, ICC and IEM.

Quick Overview for Collagen IV antibody (Biotin) (ABIN135053)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Chicken, Chinchilla chinchilla, Dog, Guinea Pig, Horse, Japanese macaque (Macaca fuscata), Sheep, Tree shrew

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Product Details anti-Collagen IV Antibody (Biotin)

Purpose: Goat Anti-Type IV Collagen-BIOT

Specificity: Reacts with conformational determinants on type IV collagen Referenced species reactivities include - Human 1,12,14,27,29,31,34,36 Chicken 5,13,15 Porcine 11,22,25 Mouse 4,5,7,8,30,32 Chinchilla 17 Rabbit 5,6,14,20,23 Rat 2,3,10,26,27,33,35 Equine 18,28 Sheep 24 Bovine 5,24 Guinea Pig 14 Tree Shrew 21 Canine 9,19 Japanese Macaque 16

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Biotin Anti-Type IV Collagen antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, western blotting, and immunoprecipitation assays.

Purification: Purified by affinity chromatography on type IV collagen covalently linked to agarose, cross-adsorbed against Collagen types I, II, III, V, and VI for minimal reactivity

Immunogen: Type IV collagen

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with type IV collagen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References for anti-Collagen IV antibody (Biotin) (ABIN135053)

Weiner, Shah, Angelopoulos, Bartakova, Pulido, Murphy, Nudleman, Daneman, Goldberg: "Cholinergic neural activity directs retinal layer-specific angiogenesis and blood retinal barrier formation." in: Nature communications, Vol. 10, Issue 1, pp. 2477, (2019) (PubMed).

Ka, Hsieh, Lin, Yang, Wu, Sytwu, Chen: "Decoy receptor 3 inhibits renal mononuclear leukocyte infiltration and apoptosis and prevents progression of IgA nephropathy in mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 6, pp. F1218-30, (2011) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Type IV Collagen

Background:

Description: Goat Anti-Type IV Collagen-BIOT

NCBI_Official_Symbol: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, COL4A6

Synoynms: COL4

Gene ID: 1282, 1284, 1285

UniProt: P02462