ITGA4 antibody

Catalog No. ABIN135079

This Rat Monoclonal antibody specifically detects ITGA4 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for ITGA4 antibody (ABIN135079)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PS-2

Product Details anti-ITGA4 Antibody

Specificity: Mouse/Human CD49d, Mr 150 kDa

Characteristics: Rat Anti-Mouse CD49d-LE/AF

Endotoxin Level: Low endotoxin

Immunogen: P815 cells

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Adhesion - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: CD49d, the 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150 kDa) VLA-4 belongs to the 1-integrin family, which functions both as cell-matrix and cell-cell receptors.1-3 As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive.

Pathways: Integrin Complex