CD22 antibody

Catalog No. ABIN135163

This Rat Monoclonal antibody specifically detects CD22 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD22 antibody (ABIN135163)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 2D6

Product Details anti-CD22 Antibody

Specificity: Mouse CD22, Mr 150 kDa

Characteristics: Rat Anti-Mouse CD22-LE/AF

Endotoxin Level: Low endotoxin

Immunogen: Splenic lymphocyte plasma membranes from CBA x C57 F1 mice

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression. CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion. Its intracellular domain is phosphorylated after antigen receptor cross-linking and is involved in negative regulation of B-cell activation.