CD25 antibody

Catalog No. ABIN135175

This Rat Monoclonal antibody specifically detects CD25 in FACS, IP, IHC (fro), BR, Neut, Dep and CMCD. It exhibits reactivity toward Mouse.

Quick Overview for CD25 antibody (ABIN135175)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Neutralization (Neut), Depletion (Dep), Complement Mediated Cell Depletion (CMCD)

Clone: 7D4

Product Details anti-CD25 Antibody

Purpose: Rat Anti-Mouse CD25-LE/AF

Specificity: Mouse CD25, Mr 55 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD25 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, blocking, depletion, CMCD, and neutralization assays.

Endotoxin Level: Low Endotoxin

Immunogen: IL-2 dependent BALB/c helper T cell clone HT2

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.

Gene ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation