CD45 antibody (FITC)

Catalog No. ABIN135221

The Rat Monoclonal anti-CD45 antibody is suitable to detect CD45 in samples from Mouse. It has been validated for FACS.

Quick Overview for CD45 antibody (FITC) (ABIN135221)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: I3-2-3

Product Details anti-CD45 Antibody (FITC)

Specificity: Mouse CD45 (all isoforms), Mr 180-240 kDa

Characteristics: Rat Anti-Mouse CD45-FITC

Immunogen: T1M1 (Thy-1-c) cells

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE, PE/TXRD, APC, SPRD, APC/CY7, PE/CY5.5, PE/CY7, 0.2 g/106 cells AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg or 0.1 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background: CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes. CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling