Bu-1b antibody

Catalog No. ABIN135582

Product Details anti-Bu-1b Antibody

Target: Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bu-1b antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Chicken Bu-1b, Mr 70 kDa

Characteristics: Mouse Anti-Chicken Bu-1b-UNLB

Purification: Purified

Immunogen: Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens

Clone: 5-11G2

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Apop - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for Bu-1b

Target: Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))

Alternative Name: Bu-1b (Bu-1b Products)

Synonyms: B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody

Background: Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.