Toxin A antibody
Reactivity: Clostridium difficile
ELISA, WB
Host: Chicken egg
unconjugated
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- Target
- Toxin A
- Reactivity
- Clostridium difficile
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Host
-
Chicken egg
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Conjugate
- Un-conjugated
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Application
- ELISA, Western Blotting (WB)
- Characteristics
- Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the immunogen immobilized on a solid support.
- Purification
- egg yolk-derived purified IgY, affinity purified
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anti-Toxin A antibody
Reactivity: Clostridium difficile ELISA, WB Host: Mouse Monoclonal unconjugated
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- Application Notes
- This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 ug of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin A. Optimal working dilutions should be determined for your particular assay conditions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.0 mg/ml
- Buffer
- Phosphate Buffered Saline (PBS), pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- Toxin A
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