TNF alpha antibody

Catalog No. ABIN1383955

This Mouse Monoclonal antibody specifically detects TNF alpha in ELISA, IHC, Func and ELISpot. It exhibits reactivity toward Human and has been mentioned in 15+ publications.

Quick Overview for TNF alpha antibody (ABIN1383955)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Functional Studies (Func), ELISpot

Clone: B-C7

Product Details anti-TNF alpha Antibody

Specificity: Recognises both natural and recombinant human TNF-a

Purification: Ion exchange chromatography

Sterility: 0.22 μm filtered

Immunogen: Recombinant human TNF-a,Myeloma X63/AG.8653 x Balb/c spleen cells

Isotype: IgG1 kappa

Biological Activity Comment: Inhibits TNFα induced cytotoxicity on U937 cells

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store at +2-8°C for 12 months. DO NOT FREEZE.

References for anti-TNF alpha antibody (ABIN1383955)

Arduise, Abache, Li, Billard, Chabanon, Ludwig, Mauduit, Boucheix, Rubinstein, Le Naour: "Tetraspanins regulate ADAM10-mediated cleavage of TNF-alpha and epidermal growth factor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 10, pp. 7002-13, (2008) (PubMed).

Venet, Pachot, Debard, Bohe, Bienvenu, Lepape, Powell, Monneret: "Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 9, pp. 6540-7, (2006) (PubMed).

Dyugovskaya, Lavie, Lavie: "Phenotypic and functional characterization of blood gammadelta T cells in sleep apnea." in: American journal of respiratory and critical care medicine, Vol. 168, Issue 2, pp. 242-9, (2003) (PubMed).

Limb, Daniels, Pleass, Charteris, Luthert, Khaw: "Differential expression of matrix metalloproteinases 2 and 9 by glial Müller cells: response to soluble and extracellular matrix-bound tumor necrosis factor-alpha." in: The American journal of pathology, Vol. 160, Issue 5, pp. 1847-55, (2002) (PubMed).

Hamacher, Lucas, Lijnen, Buschke, Dunant, Wendel, Grau, Suter, Ricou: "Tumor necrosis factor-alpha and angiostatin are mediators of endothelial cytotoxicity in bronchoalveolar lavages of patients with acute respiratory distress syndrome." in: American journal of respiratory and critical care medicine, Vol. 166, Issue 5, pp. 651-6, (2002) (PubMed).

Musey, Ding, Cao, Lee, Galloway, Yuen, Jerome, McElrath: "Ontogeny and specificities of mucosal and blood human immunodeficiency virus type 1-specific CD8(+) cytotoxic T lymphocytes." in: Journal of virology, Vol. 77, Issue 1, pp. 291-300, (2002) (PubMed).

Marfaing-Koka, Maravic, Humbert, Galanaud, Emilie: "Contrasting effects of IL-4, IL-10 and corticosteroids on RANTES production by human monocytes." in: International immunology, Vol. 8, Issue 10, pp. 1587-94, (1997) (PubMed).

Voorzanger, Touitou, Garcia, Delecluse, Rousset, Joab, Favrot, Blay: "Interleukin (IL)-10 and IL-6 are produced in vivo by non-Hodgkin's lymphoma cells and act as cooperative growth factors." in: Cancer research, Vol. 56, Issue 23, pp. 5499-505, (1997) (PubMed).

Mey, Hmama, Normier, Revillard: "Alteration of dealcylated lipopolysaccharide antagonistic properties by interaction with plasma factors." in: Journal of leukocyte biology, Vol. 61, Issue 1, pp. 17-23, (1997) (PubMed).

Ida, Robertson, Voss, Ritz, Anderson: "CD94 ligation induces apoptosis in a subset of IL-2-stimulated NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2154-60, (1997) (PubMed).

van Hensbroek, Palmer, Onyiorah, Schneider, Jaffar, Dolan, Memming, Frenkel, Enwere, Bennett, Kwiatkowski, Greenwood: "The effect of a monoclonal antibody to tumor necrosis factor on survival from childhood cerebral malaria." in: The Journal of infectious diseases, Vol. 174, Issue 5, pp. 1091-7, (1996) (PubMed).

Racadot, Milpied, Bordigoni, Cahn, Plouvier, Lioure, Lutz, Wijdenes, Herve: "Sequential use of three monoclonal antibodies in corticosteroid-resistant acute GVHD: a multicentric pilot study including 15 patients." in: Bone marrow transplantation, Vol. 15, Issue 5, pp. 669-77, (1995) (PubMed).

Boillot, Capellier, Racadot, Wijdenes, Herve, Barale: "Pilot clinical trial of an anti-TNF alpha monoclonal antibody for the treatment of septic shock." in: Clinical intensive care : international journal of critical & coronary care medicine, Vol. 6, Issue 2, pp. 52-6, (1995) (PubMed).

Hervé, Flesch, Tiberghien, Wijdenes, Racadot, Bordigoni, Plouvier, Stephan, Bourdeau, Holler: "Phase I-II trial of a monoclonal anti-tumor necrosis factor alpha antibody for the treatment of refractory severe acute graft-versus-host disease." in: Blood, Vol. 79, Issue 12, pp. 3362-8, (1992) (PubMed).

Hervé, Racadot, Wijdenes, Flesch, Tiberghien, Bordigoni, Holler, Powles, Bourdeau, Wilmer: "Monoclonal anti TNF alpha antibody in the treatment of acute GvHD refractory both to corticosteroids and anti IL-2 R antibody." in: Bone marrow transplantation, Vol. 7 Suppl 2, pp. 149, (1991) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: TNFa

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome