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Midline 2 antibody (AA 251-350)

MID2 Reactivity: Human ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1385613
  • Target See all Midline 2 (MID2) Antibodies
    Midline 2 (MID2)
    Binding Specificity
    • 14
    • 11
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 251-350
    Reactivity
    • 42
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 40
    • 2
    • 1
    Rabbit
    Clonality
    • 43
    Polyclonal
    Conjugate
    • 19
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Midline 2 antibody is un-conjugated
    Application
    • 26
    • 19
    • 18
    • 12
    • 12
    • 5
    • 2
    • 1
    • 1
    ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Cross-Reactivity
    Human
    Predicted Reactivity
    Mouse,Rat,Dog,Sheep,Pig,Horse,Chicken
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human RNF60/Midline-2
    Isotype
    IgG
    Top Product
    Discover our top product MID2 Primary Antibody
  • Application Notes
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    Midline 2 (MID2)
    Alternative Name
    RNF60/Midline-2 (MID2 Products)
    Synonyms
    MID2 antibody, fxy2 antibody, rnf60 antibody, trim1 antibody, FXY2 antibody, RNF60 antibody, TRIM1 antibody, Trim1 antibody, RGD1565674 antibody, midline 2 antibody, midline 2 L homeolog antibody, MID2 antibody, mid2 antibody, mid2.L antibody, Mid2 antibody
    Background

    Synonyms: FXY2, MID2, Midin-2, Midline 2, Midline defect 2, Midline-2, Probable E3 ubiquitin-protein ligase MID2, RING finger protein 60, Ring finger protein protein 60, RNF60, TRIM1, TRIM1_HUMAN, Tripartite mot protein 1, Tripartite mot-containing protein 1, Tripartite mot-containing protein 1, Midline defect 2, FLJ37715, FLJ41813.

    Background: Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Dimerization is mediated by the tripartite motif (RBCC, RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine.

    Gene ID
    26040
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