FBXW7 antibody (AA 501-600)

Catalog No. ABIN1386103

This Rabbit Polyclonal antibody specifically detects FBXW7 in WB, ELISA, IF (p), IHC (p), IF (cc) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for FBXW7 antibody (AA 501-600) (ABIN1386103)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FBXW7 Antibody

Binding Specificity: AA 501-600

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Fbxw7/hCDC4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FBXW7 antibody (ABIN1386103)

Wang, Yang, Liu, Huang, Wang, Chen, Chen: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).

Chen, Wang, Wang, Huang, Zhang: "STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7." in: Cancer cell international, Vol. 15, pp. 111, (2015) (PubMed).

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: Fbxw7

Background:

Synonyms: AGO, CDC4, FBW6, FBW7, hAgo, FBX30, FBXW6, SEL10, hCdc4, FBXO30, SEL-10, F-box/WD repeat-containing protein 7, Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, FBXW7

Background: Substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463).

Gene ID: 55294

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway