MPP5 antibody (AA 201-300)

Catalog No. ABIN1386594

Product Details anti-MPP5 Antibody

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Binding Specificity: AA 201-300

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MPP5/PALS1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MPP5

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Alternative Name: MPP5/PALS1 (MPP5 Products)

Synonyms: PALS1 antibody, 3830420B02Rik antibody, AI255216 antibody, AI644496 antibody, Pals1 antibody, mpp5 antibody, nok antibody, MAGUK p55 subfamily member 5 antibody, membrane palmitoylated protein 5 antibody, membrane protein, palmitoylated 5 L homeolog antibody, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) antibody, membrane protein, palmitoylated 5a (MAGUK p55 subfamily member 5) antibody, Tsp_03061 antibody, MPP5 antibody, mpp5.L antibody, Mpp5 antibody, mpp5a antibody

Background:

Synonyms: FLJ12615, MAGUK p55 subfamily member 5, Membrane protein palmitoylated 5, MPP 5, MPP5, MPP5_HUMAN, PALS 1, PALS1, Protein associated with Lin-7, Stardust Drosophila, Stardust.

Background: Two highly conserved complexes are responsible for the assembly of tight junctions, the Crumbs-Pals1-Patj complex and the Cdc42-Par6-Par3-aPKC complex. Tight junctions assist in the formation of polarity in the epithelia by establishing a barrier to separate apical and basolateral membranes. Pals1, importantly, mediates interaction between the two complexes via interaction with Par6. Loss of Pals1 function results in delayed polarization, decreased transepithelial electrical resistance and an inability to form lumenal cysts. Because tumors exhibit perturbations in epithelial polarity, Pals1 presents a new potential target in the study of carcinogenesis.

Pathways: Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization