Acrosin antibody (AA 40-120)

Catalog No. ABIN7212412

The Rabbit Polyclonal anti-Acrosin antibody is suitable to detect Acrosin in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Acrosin antibody (AA 40-120) (ABIN7212412)

Target: Acrosin (ACR)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acrosin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Acrosin Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-Acrosin Polyclonal Antibody

Specificity: Acrosin Polyclonal Antibody detects endogenous levels of Acrosin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Acrosin at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Acrosin

Target: Acrosin (ACR)

Alternative Name: Acrosin

Background: ACR, ACRS, AcrosinAcrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Molecular Weight: 45kD

Gene ID: 49

UniProt: P10323

Pathways: cAMP Metabolic Process