PPP1R9A antibody (AA 115-165)

Catalog No. ABIN1387768

Product Details anti-PPP1R9A Antibody

Target: PPP1R9A (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9A (PPP1R9A))

Binding Specificity: AA 115-165

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R9A antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Neurabin 1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PPP1R9A

Target: PPP1R9A (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9A (PPP1R9A))

Alternative Name: Neurabin 1/PPP1R9A (PPP1R9A Products)

Synonyms: 2810430P21Rik antibody, 4930518N04Rik antibody, 5330407E15 antibody, A230094E16Rik antibody, BB181831 antibody, NRB antibody, mKIAA1222 antibody, neurabin-I antibody, NRB1 antibody, NRBI antibody, Neurabin-I antibody, Neb1 antibody, protein phosphatase 1, regulatory (inhibitor) subunit 9A antibody, protein phosphatase 1 regulatory subunit 9A antibody, protein phosphatase 1, regulatory subunit 9A antibody, Ppp1r9a antibody, PPP1R9A antibody

Background:

Synonyms: FLJ20068, KIAA1222, NEB1_HUMAN, Neurabin I, Neurabin-1, Neurabin-I, Neurabin1, Neurabin-1, NeurabinI, Neurabin1, Neural tissue specic F actin binding protein I, Neural tissue-specic F-actin-binding protein I, NRB 1, NRB I, NRB1, NRBI, PPP1R9A, Protein phosphatase 1 regulatory inhibitor subunit 9A, Protein phosphatase 1 regulatory subunit 9A.

Background: Brain-specific neurabin I (neural tissue-specific F-actin binding protein I) is highly concentrated in the synapse of developed neurons, it localizes in the growth cone lamellipodia during neuronal development (1). Suppression of endogenous neurabin in rat hippocampal neurons inhibits neurite formation (1). Neurabin I recruits active PP1 via a PP1-docking sequence, mutation of the PP1-binding motif halts filopodia and restores stress fibers in neurabin I-expressing cells (2,3). Neurabin II (Spinophilin) is ubiquitously expressed but is abundantly expressed in brain (4). Neurabin II localizes to neuronal dentritic spines, which are the specialized protrusions from dendritic shafts that receive most of the excitatory input in the CNS (5). Neurabin II may regulate dendritic spine properties as neurabin II(-) mice have increased spine density during development in vitro and exhibit altered filopodial formation in cultured cells (5). Neurabin may also play a role in glutamatergic transmission as Neurabin II(-) mice exhibit reduced long-term depression and resistance to kainate-induced seizures and neronal apoptosis (5). Neurabin II complexes with the catalytic subunit of protein phosphatase-1 (PP1) in vitro thus modulating the activity of PP1 (4).