MYF5 antibody (AA 61-160)

Catalog No. ABIN1387888

This Rabbit Polyclonal antibody specifically detects MYF5 in ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for MYF5 antibody (AA 61-160) (ABIN1387888)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MYF5 Antibody

Binding Specificity: AA 61-160

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MYF5

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: MYF5

Background:

Synonyms: Myf-5, bHLHc2, Class C basic helix loop helix protein 2, Class C basic helix-loop-helix protein 2, Myf 5, Myf-5, Myf5, MYF5_HUMAN, Myogenic factor 5.

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Gene ID: 4617

UniProt: P13349

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development