CHEK1 antibody (pSer296) (AbBy Fluor® 647)

Catalog No. ABIN1390020

This anti-CHEK1 antibody is a Rabbit Polyclonal antibody detecting CHEK1 in WB, IF (cc) and IF (p). Suitable for Human.

Quick Overview for CHEK1 antibody (pSer296) (AbBy Fluor® 647) (ABIN1390020)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CHEK1 Antibody (AbBy Fluor® 647)

Binding Specificity: pSer296

Cross-Reactivity: Human

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CHEK1 isoform b around the phosphorylation site of Ser296

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1

Background:

Synonyms: Chk1 Ser296, Chk1 phospho S296,Chk1 Ser296, p-Chk1 phospho S296, Chk1S296, Cell cycle checkpoint kinase, Checkpoint, S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog S. pombe, CHEK 1, CHEK-1, CHEK1, Chk 1, Chk1, CHK1 checkpoint homolog S. pombe, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A S. cerevisiae, C85740, CHK1_HUMAN.

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011].

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair