LIM Domain Kinase 1 antibody (AA 365-470) (Biotin)

Catalog No. ABIN1393819

Product Details anti-LIM Domain Kinase 1 Antibody (Biotin)

Target: LIM Domain Kinase 1 (LIMK1)

Binding Specificity: AA 365-470

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LIM kinase 1 + 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIM kinase 1 (LIMK1 Products)

Synonyms: limk1 antibody, xlimk1 antibody, GB16654 antibody, LIMK1 antibody, LIMK antibody, LIMK-1 antibody, LIM domain kinase 1 antibody, LIM domain kinase 1a antibody, LIM domain kinase 1 L homeolog antibody, LIM-domain containing, protein kinase antibody, LIMK1 antibody, limk1a antibody, limk1 antibody, CpipJ_CPIJ000717 antibody, LOC413152 antibody, Limk1 antibody, limk1.L antibody

Background:

Synonyms: LIM kinase 1+2, LIMK1+LIMK2, LIM domain kinase 1, LIM domain kinase 2, LIMK1, LIMK2,LIMK1_HUMAN, LIMK2_HUMAN.

Background: LIMK 1 and 2 likely regulate aspects of the cytoskeleton, through control of the organization of actin filaments. They can phosphorylate an actin-binding protein, cofilin which binds to actin monomers and polymers and promotes the disassembly of actin filament.The phosphorylation of cofilin via LIMK inactivates this potential. LIMK1 is highly active in the brain and spinal chord, where it is believed to be involved in the development of nerve cells whilst LIMK2 is ubiquitously expressed in many adult tissues. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). These parts of the brain are important for visualizing an object as a set of parts and performing tasks such as writing, drawing, constructing models, and assembling puzzles. LIMK1 is specifically stimulated by Rac, one of the Rho family proteins, while LIMK2 activity is activated under the control of other Rho family members, Rho and Cdc42, suggesting that two distinct pathways exist in the Rho family driven actin cytoskeleton dynamics.

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events