Dihydrofolate Reductase antibody (AbBy Fluor® 350)

Catalog No. ABIN1400589

The AbBy Fluor® 350-conjugated Rabbit Polyclonal anti-Dihydrofolate Reductase antibody (ABIN1400589) specifically detects Dihydrofolate Reductase in IF. The antibody is reactive with Human samples.

Quick Overview for Dihydrofolate Reductase antibody (AbBy Fluor® 350) (ABIN1400589)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is conjugated to AbBy Fluor® 350

Application: Immunofluorescence (IF)

Product Details anti-Dihydrofolate Reductase Antibody (AbBy Fluor® 350)

Purpose: DHFR Polyclonal Antibody, AbBy Fluor-350 Conjugated

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DHFR

Isotype: IgG

Application Details

Application Notes: IF(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background:

Synonyms: DHFR, DHFRP1, Dihydrofolate reductase, DYR, DYR_HUMAN, EC 1.5.1.3.

Background: DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Molecular Weight: 21kDa

Gene ID: 1719

HGNC: 0

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases