MED7 antibody (AA 101-200) (HRP)

Catalog No. ABIN1409108

Product Details anti-MED7 Antibody (HRP)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Binding Specificity: AA 101-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED7 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CRSP9

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Alternative Name: CRSP9 (MED7 Products)

Synonyms: ARC34 antibody, CRSP33 antibody, CRSP9 antibody, 1110063B05Rik antibody, Crsp33 antibody, Crsp9 antibody, crsp9 antibody, wu:fd99d12 antibody, zgc:56241 antibody, crsp9-A antibody, med7-a antibody, mediator complex subunit 7 antibody, mediator complex subunit 7 L homeolog antibody, mediator complex subunit antibody, mediator complex subunit Med7 antibody, MED7 antibody, Med7 antibody, med7 antibody, med7.L antibody

Background:

Synonyms: Cofactor required for Sp1 transcriptional activation subunit 9, CRSP complex subunit 9, CRSP33, hMED7, MED7, MED7_HUMAN, Mediator complex subunit 7, Mediator of RNA polymerase II transcription subunit 7, MGC12284, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33, Activator recruited cofactor 34 kDa component, Activator-recruited cofactor 34 kDa component, ARC34.

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase (1). These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes, and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34) (2,3). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors (4). Med6 and Med7, as well as other components of SMCC, associate with coactivator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation (4,5). Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity (6).

Gene ID: 9443

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha