PIGA antibody (HRP)

Catalog No. ABIN1421098

This Rabbit Polyclonal antibody specifically detects PIGA in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIGA antibody (HRP) (ABIN1421098)

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIGA Antibody (HRP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PIGA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: PIGA

Background:

Synonyms: GlcNAc PI synthesis protein, GlcNAc-PI synthesis protein, GPI3, Phosphatidylinositol glycan anchor biosynthesis class A, phosphatidylinositol glycan anchor biosynthesis, class A paroxysmal nocturnal hemoglobinuria, Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, PIG A, PIG-A, PIGA_HUMAN, Phosphatidylinositol-glycan biosynthesis class A protein, PIG-A.

Background: PIGA belongs to the glycosyltransferase 1 family and is necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis. Defects in PIGA are the cause of paroxysmal nocturnal hemoglobinuria (PNH) which is an acquired hemolytic blood disorder characterized by chronic hemolysis with hemoglobinuria, increased tendency to venous thrombosis, and variable degrees of bone marrow failure.

Gene ID: 5277

Pathways: Inositol Metabolic Process