GFP antibody

Catalog No. ABIN1439998

This Goat Polyclonal antibody specifically detects GFP in WB, IF, IHC (fro) and IHC (p). It exhibits reactivity toward Aequorea aequorea and has been mentioned in 18+ publications.

Quick Overview for GFP antibody (ABIN1439998)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea aequorea

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFP Antibody

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

Characteristics: Green Fluorescent Protein

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GFP antibody (ABIN1439998)

Panayiotou, Michael, Zaravinos, Demirag, Achilleos, Strati: "Human papillomavirus E7 binds Oct4 and regulates its activity in HPV-associated cervical cancers." in: PLoS pathogens, Vol. 16, Issue 4, pp. e1008468, (2020) (PubMed).

Wu, Yi, Manca, Javaid, Lauer, Glowatzki: "Sound exposure dynamically induces dopamine synthesis in cholinergic LOC efferents for feedback to auditory nerve fibers." in: eLife, Vol. 9, (2020) (PubMed).

Fukuda, Kaneshige, Kaji, Noguchi, Takemoto, Zhang, Tsujikawa, Kokubo, Uezumi, Maehara, Harada, Ohkawa, Fukada: "Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle." in: eLife, Vol. 8, (2020) (PubMed).

Zhang, Uezumi, Kaji, Tsujikawa, Andersen, Jensen, Fukada: "Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration." in: International journal of molecular sciences, Vol. 20, Issue 13, (2019) (PubMed).

Vyas, Wu, Jimenez, Glowatzki, Fuchs: "Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea." in: Scientific reports, Vol. 9, Issue 1, pp. 5549, (2019) (PubMed).

Larson, Mironova, Vanderpool, Waisman, Rash, Agarwal, Bergles: "Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility." in: eLife, Vol. 7, (2019) (PubMed).

Ramos-Nascimento, Kellen, Ferreira, Alenquer, Vale-Costa, Raposo, Delevoye, Amorim: "KIF13A mediates trafficking of influenza A virus ribonucleoproteins." in: Journal of cell science, Vol. 130, Issue 23, pp. 4038-4050, (2018) (PubMed).

Wu, Vyas, Glowatzki, Fuchs: "Opposing expression gradients of calcitonin-related polypeptide alpha (Calca/Cgrpα) and tyrosine hydroxylase (Th) in type II afferent neurons of the mouse cochlea." in: The Journal of comparative neurology, Vol. 526, Issue 3, pp. 425-438, (2018) (PubMed).

Festa, Chen, Berquez, Debaix, Tokonami, Prange, Hoek, Alessio, Raimondi, Nevo, Giles, Devuyst, Luciani: "Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney." in: Nature communications, Vol. 9, Issue 1, pp. 161, (2018) (PubMed).

Mimura, Takagi, Clever, Imamoto: "ELYS regulates the localization of LBR by modulating its phosphorylation state." in: Journal of cell science, Vol. 129, Issue 22, pp. 4200-4212, (2017) (PubMed).

Vale-Costa, Alenquer, Sousa, Kellen, Ramalho, Tranfield, Amorim: "Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors." in: Journal of cell science, Vol. 129, Issue 8, pp. 1697-710, (2017) (PubMed).

Agarwal, Wu, Hughes, Fukaya, Tischfield, Langseth, Wirtz, Bergles: "Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes." in: Neuron, Vol. 93, Issue 3, pp. 587-605.e7, (2017) (PubMed).

Vyas, Wu, Zimmerman, Fuchs, Glowatzki: "Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice." in: Journal of the Association for Research in Otolaryngology : JARO, Vol. 18, Issue 1, pp. 139-151, (2017) (PubMed).

Martins-Marques, Anjo, Pereira, Manadas, Girão: "Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 11, pp. 3040-55, (2016) (PubMed).

Sargiannidou, Kim, Kyriakoudi, Eun, Kleopa: "A start codon CMT1X mutation associated with transient encephalomyelitis causes complete loss of Cx32." in: Neurogenetics, Vol. 16, Issue 3, pp. 193-200, (2016) (PubMed).

Kourti, Ikonomou, Giakoumakis, Rapsomaniki, Landegren, Siniossoglou, Lygerou, Simos, Mylonis: "CK1δ restrains lipin-1 induction, lipid droplet formation and cell proliferation under hypoxia by reducing HIF-1α/ARNT complex formation." in: Cellular signalling, Vol. 27, Issue 6, pp. 1129-40, (2015) (PubMed).

Paukert, Agarwal, Cha, Doze, Kang, Bergles: "Norepinephrine controls astroglial responsiveness to local circuit activity." in: Neuron, Vol. 82, Issue 6, pp. 1263-70, (2014) (PubMed).

Pais, Milho, Almeida, Mota: "Identification of novel type III secretion chaperone-substrate complexes of Chlamydia trachomatis." in: PLoS ONE, Vol. 8, Issue 2, pp. e56292, (2013) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Synonyms: Green fluorescent protein antibody.