Connexin 43/GJA1 antibody (C-Term)

Catalog No. ABIN1440034

This Goat Polyclonal antibody specifically detects Connexin 43/GJA1 in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Dog, Monkey, Cow, Rabbit, Chicken, Pig, Guinea Pig, Horse, Sheep, Hamster, Goat, Avian, Cat and Donkey. It has been mentioned in 3+ publications

Quick Overview for Connexin 43/GJA1 antibody (C-Term) (ABIN1440034)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Rabbit, Chicken, Pig, Guinea Pig, Horse, Sheep, Hamster, Goat, Avian, Cat, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: C-Term

Purpose: Anti-CX43

Sequence: VKDRVKGRSD PYHATTGPLS PSKDCGSPKY AYFNGCSSPT APLSPMSPPG YKLVTGDRNN SSCRNYNKQA SEQNWANYSA EQNRMGQAGS TISNSHAQPF DFPDDNQNAK KVAAGHELQP LAIVDQRPSS RASSRASSRP RPDDLEI

Specificity: Detects endogenous levels of total connexin 43.

Characteristics: Gap junction protein, alpha 1, 43 kDa

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:250-1:800, IHC-F:1:250-1:800

Restrictions: For Research Use only

Handling

Concentration: 4 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-Connexin 43/GJA1 antibody (ABIN1440034)

Martins-Marques, Pinho, Zuzarte, Oliveira, Pereira, Sluijter, Gomes, Girao: "Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin." in: Journal of extracellular vesicles, Vol. 5, pp. 32538, (2016) (PubMed).

Martins-Marques, Anjo, Pereira, Manadas, Girão: "Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 11, pp. 3040-55, (2016) (PubMed).

Ribeiro-Rodrigues, Catarino, Marques, Ferreira, Martins-Marques, Pereira, Girão: "AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 11, pp. 4629-41, (2015) (PubMed).

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: CX43

Background:

Goat polyclonal to connexin 43, which is a member of the connexin family. Cx43 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. This protein is the major protein of gap junctions in embryonic development and in the heart that are thought to have a crucial role in the synchronized contraction of the heart.

AVSD3, CX43, DFNB38, gap junction protein, alpha 1, 43 kDa, GJA1, GJAL HSS, HLHS1, ODDD antibody.

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization