p53 antibody (pSer20)

Catalog No. ABIN1449251

The Mouse Monoclonal anti-p53 antibody has been validated for WB. It is suitable to detect p53 in samples from Human.

Quick Overview for p53 antibody (pSer20) (ABIN1449251)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 17B6

Product Details anti-p53 Antibody

Binding Specificity: pSer20

Specificity: This antibody reacts with the human phosphorylated p53 (Ser20).

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 50 μg IgG in 50 μL volume of PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53)

Background: P53 mutants are found in more than half of human cancers and ar e considered as the most important human cancer related gene. p53 is detected at 53 kDa position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by the phosphorylation of serine and threonine, and th e acetylation of lysine at various sites in the molecule (Ref 1). p53 at Ser20 is phosphorylated by Chk1 and Chk2 kinases and this enhances tetramerization, stability and activity of p53 (Ref 2,3)Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gene ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect