p53 antibody (pSer315)

Catalog No. ABIN1449252

This anti-p53 antibody is a Rat Monoclonal antibody detecting p53 in WB. Suitable for Human.

Quick Overview for p53 antibody (pSer315) (ABIN1449252)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: #18

Product Details anti-p53 Antibody

Binding Specificity: pSer315

Specificity: This antibody reacts with the human phosphorylated p53 (Ser315).

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store (in aliqouts) at -20 °C.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53)

Background: P53 mutants are found in more than half of human cancers and ar e considered as the most important human cancer related gene. p53 is detected at 53 kDa position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by the phosphorylation of serine and threonine, and the acetylation of lysine at various sites in the molecule. Ser315 is phosphorylated by aurora kinase and cyclin-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole Ref. 1-3 . However the effect of the phosphorylation on the function of p53 is largely unknown.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Gene ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect