GRB2 antibody (N-Term)

Catalog No. ABIN1449302

The Mouse Monoclonal anti-GRB2 antibody is suitable to detect GRB2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for GRB2 antibody (N-Term) (ABIN1449302)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRB2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3F2

Product Details anti-GRB2 Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Ammonium sulfate precipitation and affinity chromatography on protein A

Immunogen: Recombinant Ash/Grb-2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 100 μL of distilled water.

Buffer: PBS, 1 mg/mL Sodium azide, 10 mg/mL sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted (in aliquots) at -20 °C.

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2 / ASH

Background: Grb2 (Growth factor receptor bound protein 2) also known as Ash, is a 25-28 kDa molecule composed of central Src homology (SH2) domain and SH3 domains. Grb2 associates with tyrosine phosphorylated proteins such as EGFR, PDGFR, IRS-1, Shc and Gab1 via its Sh2 domain. The SH3 domain of Grb2 binds to Sos, which stimulates the GTP binding activity of Ras, cause activation of MAP kinase and other signaling pathways. Grb2 is an adaptor protein, which does not have enzymatic activity itself. Grb2 express ubiquitously and has two isoforms.Synonyms: Growth factor receptor-bound protein 2

Gene ID: 2885

NCBI Accession: NP_002077

UniProt: P62993

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation