mLC-2 (AA 5-54), (pThr17) antibody
Reactivity: Human, Mouse, Rat
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
(1 reference)-
- Target
- mLC-2
-
Binding Specificity
- AA 5-54, pThr17
- Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
-
Application
- ELISA, Immunohistochemistry (IHC)
- Specificity
-
MLC-2 (Phospho-Thr17+Ser18) Antibody detects endogenous levels of MLC-2 only when phosphorylated at Thr17 and Ser18.
PhosphorylationH:T17+S18, M:T19+S20, R:T18+S19
- Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
- Immunogen
- The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18.
- Isotype
- IgG
-
-
- Application Notes
- IHC: 1:50~1:100 ELISA: 1:10000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Stable at -20°C for at least 1 year.
-
-
: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).
-
: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).
-
- Target
- mLC-2
- Synonyms
- 2410014J02Rik antibody, MLC-2 antibody, Mlc2 antibody, Mrlc2 antibody, Mrlcb antibody, Mylc2b antibody, CMH10 antibody, MLC2 antibody, myosin light chain, phosphorylatable, fast skeletal muscle antibody, myosin light chain 12B antibody, myosin light chain 2 antibody, Mylpf antibody, Myl12b antibody, MYL2 antibody
- Background
-
Synonyms: MLCB, MLRA, MLRM, MRCL3, Myosin RLC, Myosin RLC-A, Myosin regulatory light chain, Myosin regulatory light chain 2, nonsarcomeric, Myosin
NCBI Gene Symbol: MYL12A
- Molecular Weight
- 19 kDa
- Gene ID
- 10627
- OMIM
- 609211
- UniProt
- P19105, O14950, P24844
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