FADD antibody (pSer191)

Catalog No. ABIN1450524

The Rabbit Polyclonal anti-FADD antibody (ABIN1450524) specifically detects FADD in WB, IHC, ELISA and IF. The antibody is reactive with Mouse samples.

Quick Overview for FADD antibody (pSer191) (ABIN1450524)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-FADD Antibody

Binding Specificity: AA 157-206, pSer191

Purpose: FADD (phospho Ser191) Antibody

Specificity: Phospho-FADD (S191) Polyclonal Antibody detects endogenous levels of FADD protein only when phosphorylated at S191.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from mouse FADD around the phosphorylation site of Ser191. AA range:157-206

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD

Background:

Synonyms: FADD, MORT1, GIG3, Protein FADD, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity

Background: The Fas associated via death domain encoded by FADD is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Gene Name: FADD

Protein Name: Protein FADD

Molecular Weight: 23 kDa

UniProt: Q13158

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades