GCK antibody (AA 43-92)

Catalog No. ABIN1453323

The Rabbit Polyclonal anti-GCK antibody (ABIN1453323) specifically detects GCK in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for GCK antibody (AA 43-92) (ABIN1453323)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GCK Antibody

Binding Specificity: AA 43-92

Purpose: GCK Antibody

Specificity: GCK Polyclonal Antibody detects endogenous levels of GCK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human GCK. AA range:43-92

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK

Background:

Synonyms: GCK, Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009],

Gene Name: GCK

Protein Name: Glucokinase

Molecular Weight: 52 kDa

Gene ID: 2645

OMIM: 138079

UniProt: P35557

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process