Cytochrome b antibody (N-Term)

Catalog No. ABIN6749746

The Rabbit Polyclonal anti-Cytochrome b antibody (ABIN6749746) specifically detects Cytochrome b in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Cytochrome b antibody (N-Term) (ABIN6749746)

Target: Cytochrome b (MT-CYB) (Mitochondrially Encoded Cytochrome B (MT-CYB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome b antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cytochrome b Antibody

Binding Specificity: N-Term

Specificity: Human MT-CYB

Predicted Reactivity: Percent identity by BLAST analysis: Hamster (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CYTB. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Platypus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Cytochrome b

Target: Cytochrome b (MT-CYB) (Mitochondrially Encoded Cytochrome B (MT-CYB))

Alternative Name: CYTB / MT-CYB

Background: Name/Gene ID: MT-CYB

Synonyms: MT-CYB, COB, CYTB, Complex III subunit III, Cytochrome b, MTCYB, UQCR3, Complex III subunit 3

Gene ID: 4519

UniProt: P00156

Pathways: Proton Transport