Actin antibody

Catalog No. ABIN1502284

This Mouse Monoclonal antibody specifically detects Actin in WB, ELISA, IHC, IHC (p), IHC (fro) and IEM. It exhibits reactivity toward Human, Rat, Zebrafish (Danio rerio), Rabbit, Pig, Chicken and Monkey.

Quick Overview for Actin antibody (ABIN1502284)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Rat, Zebrafish (Danio rerio), Rabbit, Pig, Chicken, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)

Clone: HHF35

Product Details anti-Actin Antibody

Specificity: Reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections. The epitope recognized by HHF35 is highly conserved. Therefore the antibody cross-reacts with several species including human, monkey, swine, rabbit, rat, chicken and zebrafish

Purification: Protein G purified

Immunogen: SDS-extracted protein fraction from human myocardium

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, EM, IHC, IHC-Fr (1:200 - 1:1000), IHC-P (5 μg/mL), WB (1:1000 - 1:5000)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Muscle Actin

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development