BRCA1 antibody (AA 120-220)

Catalog No. ABIN1512646

This Rabbit Polyclonal antibody specifically detects BRCA1 in WB and IHC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for BRCA1 antibody (AA 120-220) (ABIN1512646)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 120-220

Sequence: DEVSIIQSMG YRNRAKRLLQ SEPENPSLQE TSLSVQLSNL GTVRTLRTKQ RIQPQKTSVY IELGSDSSED TVNKATYCSV GDQELLQITP QGTRDEISLD S

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 120-220 of human BRCA1 (NP_009225.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-BRCA1 antibody (ABIN1512646)

Yin, Liu, Peng, Peng, Yu, Gao, Yuan, Zhu, Cao, He, Gong, Sun, Fan, Li: "PARP inhibitor veliparib and HDAC inhibitor SAHA synergistically co-target the UHRF1/BRCA1 DNA damage repair complex in prostate cancer cells." in: Journal of experimental & clinical cancer research : CR, Vol. 37, Issue 1, pp. 153, (2018) (PubMed).

Penn, Yang, Zong, Lim, Cole, Yang, Baker, Goonewardena, Buckanovich: "Therapeutic Impact of Nanoparticle Therapy Targeting Tumor-Associated Macrophages." in: Molecular cancer therapeutics, Vol. 17, Issue 1, pp. 96-106, (2018) (PubMed).

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.,BRCA1,BRCAI,BRCC1,BROVCA1,FANCS,IRIS,PNCA4,PPP1R53,PSCP,RNF53,Epigenetics & Nuclear Signaling,DNA Damage & Repair,Cancer,Tumor suppressors,Cell Biology & Developmental Biology,Cell Cycle,Centrosome,G2/M DNA Damage Checkpoint,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,BRCA1

Molecular Weight: 7 kDa/78-85 kDa/202-210 kDa

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus