AMPK alpha antibody (pThr172)

Catalog No. ABIN1531172

The Rabbit Polyclonal anti-AMPK alpha antibody (ABIN1531172) specifically detects AMPK alpha in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse, Rat, Monkey, Pig and Shrimp samples.

Quick Overview for AMPK alpha antibody (pThr172) (ABIN1531172)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Shrimp

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-AMPK alpha Antibody

Binding Specificity: AA 140-189, pThr172

Purpose: AMPKα1/2 (phospho Thr183/172) Antibody

Specificity: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Isotype: IgG

Application Details

Application Notes: IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: AMPK alpha

Background:

Synonyms: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Gene Name: AAPK1/AAPK2

Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2

Molecular Weight: 62 kDa

Gene ID: 5562, 5563

OMIM: 602739

UniProt: Q13131

Pathways: Warburg Effect