CHEK2 antibody (pThr387)

Catalog No. ABIN1531263

This Rabbit Polyclonal antibody specifically detects CHEK2 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CHEK2 antibody (pThr387) (ABIN1531263)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CHEK2 Antibody

Binding Specificity: AA 361-410, pThr387

Purpose: Chk2 (phospho Thr387) Antibody

Specificity: Phospho-Chk2 (T387) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T387.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr387. AA range:361-410

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: Chk2

Background:

Synonyms: CHEK2, CDS1, CHK2, RAD53, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators.The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati

Gene Name: CHEK2

Protein Name: Serine/threonine-protein kinase Chk2

Molecular Weight: 60 kDa

Gene ID: 11200

OMIM: 176807

UniProt: O96017

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle