Cyclin D3 antibody (pThr283)

Catalog No. ABIN1531266

This Rabbit Polyclonal antibody specifically detects Cyclin D3 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin D3 antibody (pThr283) (ABIN1531266)

Target: Cyclin D3 (CCND3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Cyclin D3 Antibody

Binding Specificity: AA 243-292, pThr283

Purpose: Cyclin D3 (phospho Thr283) Antibody

Specificity: Phospho-Cyclin D3 (T283) Polyclonal Antibody detects endogenous levels of Cyclin D3 protein only when phosphorylated at T283.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D3 around the phosphorylation site of Thr283. AA range:243-292

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Cyclin D3

Target: Cyclin D3 (CCND3)

Alternative Name: Cyclin D3

Background:

Synonyms: CCND3, G1/S-specific cyclin-D3

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008],

Gene Name: CCND3

Protein Name: G1/S-specific cyclin-D3

Molecular Weight: 32 kDa

Gene ID: 896

OMIM: 123834

UniProt: P30281

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process