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Bcl-2 antibody (pSer87)

This Rabbit Polyclonal antibody specifically detects Bcl-2 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.
Catalog No. ABIN1531302

Quick Overview for Bcl-2 antibody (pSer87) (ABIN1531302)

Target

See all Bcl-2 (BCL2) Antibodies
Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity

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  • 170
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  • 37
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Human, Mouse, Rat

Host

  • 361
  • 151
  • 4
  • 2
  • 1
Rabbit

Clonality

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  • 187
Polyclonal

Conjugate

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  • 8
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This Bcl-2 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Binding Specificity

    • 55
    • 36
    • 26
    • 23
    • 22
    • 21
    • 16
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    • 16
    • 11
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 61-110, pSer87

    Specificity

    BCL-2 (Phospho-Ser87) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Ser87.

    PhosphorylationH:S87 M:S84 R:S84

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Ser87.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

    Comment

    Unigene-Number: Hs.150749 (NCBI Gene Symbol: BCL2)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

    Alternative Name

    BCL-2

    Background

    Synonyms: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, BCL2

    NCBI Gene Symbol: BCL2

    Molecular Weight

    26 kDa

    Gene ID

    10000

    OMIM

    151430

    UniProt

    P10415

    Pathways

    MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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