Calnexin antibody (pSer583)

Catalog No. ABIN1531305

This Rabbit Polyclonal antibody specifically detects Calnexin in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Calnexin antibody (pSer583) (ABIN1531305)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-Calnexin Antibody

Binding Specificity: AA 543-592, pSer583

Purpose: Calnexin (phospho Ser583) Antibody

Specificity: Phospho-Calnexin (S583) Polyclonal Antibody detects endogenous levels of Calnexin protein only when phosphorylated at S583.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Calnexin around the phosphorylation site of Ser583. AA range:543-592

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background:

Synonyms: CANX, Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90

Background: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008],

Gene Name: CANX

Protein Name: Calnexin

Molecular Weight: 67 kDa

Gene ID: 821

OMIM: 114217

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis