FOXO1 antibody (pSer329)

Catalog No. ABIN1531321

The Rabbit Polyclonal anti-FOXO1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect FOXO1 in samples from Human, Mouse and Rat.

Quick Overview for FOXO1 antibody (pSer329) (ABIN1531321)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FOXO1 Antibody

Binding Specificity: AA 295-344, pSer329

Specificity:

FOXO1A (Phospho-Ser329) Antibody detects endogenous levels of FOXO1A only when phosphorylated at Ser329.

PhosphorylationH:S329 M:S326 R:S323

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FOXO1A around the phosphorylation site of Ser329.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.370666 (NCBI Gene Symbol: FOXO1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1A

Background:

Synonyms: FKHR, forkhead box O1A (FKHR), Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1

NCBI Gene Symbol: FOXO1

Molecular Weight: 69 kDa

Gene ID: 2308

OMIM: 136533

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling