PPP1R12A antibody (pThr696)

Catalog No. ABIN1531348

The Rabbit Polyclonal anti-PPP1R12A antibody has been validated for IHC, ELISA and IF. It is suitable to detect PPP1R12A in samples from Human, Mouse and Rat.

Quick Overview for PPP1R12A antibody (pThr696) (ABIN1531348)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PPP1R12A Antibody

Binding Specificity: AA 661-710, pThr696

Specificity:

MYPT1 (Phospho-Thr696) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Thr696.

PhosphorylationH:T696 M:T694 R:T697

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr696.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

Comment:

Unigene-Number: Hs.49582 (NCBI Gene Symbol: PPP1R12A)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: MYPT1

Background:

Synonyms: MBS, Myosin phosphatase target subunit 1, PPP1R12A

NCBI Gene Symbol: PPP1R12A

Molecular Weight: 115 kDa

Gene ID: 4659

OMIM: 602021

UniProt: O14974

Pathways: M Phase