RPS6 antibody (pSer6, pSer240)

Catalog No. ABIN1531390

The Rabbit Polyclonal anti-RPS6 antibody is suitable to detect RPS6 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for RPS6 antibody (pSer6, pSer240) (ABIN1531390)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RPS6 Antibody

Binding Specificity: AA 200-249, pSer6, pSer240

Purpose: Ribosomal Protein S6 (phospho Ser240) Antibody

Specificity: Phospho-Ribosomal Protein S6 (S240) Polyclonal Antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at S240.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human S6 Ribosomal Protein around the phosphorylation site of Ser240. AA range:200-249

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: S6 Ribosomal Protein

Background:

Synonyms: RPS6, OK/SW-cl.2, 40S ribosomal protein S6, Phosphoprotein NP33

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed

Gene Name: RPS6

Protein Name: 40S ribosomal protein S6

Molecular Weight: 28 kDa

Gene ID: 6194

OMIM: 180460

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly