CHEK1 antibody (pSer296)

Catalog No. ABIN1531427

The Rabbit Polyclonal anti-CHEK1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect CHEK1 in samples from Human.

Quick Overview for CHEK1 antibody (pSer296) (ABIN1531427)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 266-315, pSer296

Specificity:

Chk1 (Phospho-Ser296) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser296.

PhosphorylationH:S296

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser296.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1

Background:

Synonyms: CHEK1, Chk1, kinase Chk1

NCBI Gene Symbol: CHK1

Molecular Weight: 54 kDa

Gene ID: 1111

OMIM: 603078

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair