PKC mu antibody (pSer738, pSer742)

Catalog No. ABIN1531466

The Rabbit Polyclonal anti-PKC mu antibody is suitable to detect PKC mu in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for PKC mu antibody (pSer738, pSer742) (ABIN1531466)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC mu antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PKC mu Antibody

Binding Specificity: AA 706-755, pSer738, pSer742

Purpose: PKD1/2/3 (phospho Ser738/S742) Antibody

Specificity: Phospho-PKD1/2/3 (S738/S742) Polyclonal Antibody detects endogenous levels of PKD1/2/3 protein only when phosphorylated at S738/S742.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKD1/2/3/PKC mu around the phosphorylation site of Ser738 and Ser742. AA range:706-755

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Background:

Synonyms: PRKD1, PKD, PKD1, PRKCM, Serine/threonine-protein kinase D1, Protein kinase C mu type, Protein kinase D, nPKC-D1, nPKC-mu, PRKD2, PKD2, HSPC187, Serine/threonine-protein kinase D2, nPKC-D2, PRKD3, EPK2, PRKCN, Serine/threonine-protein kinas

Background: PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],

Gene Name: KPCD1/KPCD2/KPCD3

Protein Name: Serine/threonine-protein kinase D1/2/3

Molecular Weight: 101 kDa

Gene ID: 5587, 25865, 23683

OMIM: 605435

UniProt: Q15139

Pathways: Myometrial Relaxation and Contraction