BLM antibody (pThr99)

Catalog No. ABIN1531494

The Rabbit Polyclonal anti-BLM antibody (ABIN1531494) specifically detects BLM in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BLM antibody (pThr99) (ABIN1531494)

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BLM Antibody

Binding Specificity: AA 65-114, pThr99

Purpose: BLM (phospho Thr99) Antibody

Specificity: Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99. AA range:65-114

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Background:

Synonyms: BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3

Background: The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008],

Gene Name: BLM

Protein Name: Bloom syndrome protein

Molecular Weight: 159 kDa

Gene ID: 641

OMIM: 210900

UniProt: P54132

Pathways: DNA Damage Repair