Retinoblastoma Protein (Rb) antibody (pThr821)

Catalog No. ABIN1531516

This Rabbit Polyclonal antibody specifically detects Retinoblastoma Protein (Rb) in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Retinoblastoma Protein (Rb) antibody (pThr821) (ABIN1531516)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma Protein (Rb) antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Retinoblastoma Protein (Rb) Antibody

Binding Specificity: AA 791-840, pThr821

Purpose: Rb (phospho Thr821) Antibody

Specificity: Phospho-Rb (T821) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at T821.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr821. AA range:791-840

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Background:

Synonyms: RB1, Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110

Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],

Gene Name: RB1

Protein Name: Retinoblastoma-associated protein

Molecular Weight: 106 kDa

Gene ID: 5925

OMIM: 109800

UniProt: P06400

Pathways: Mitotic G1-G1/S Phases